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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2178 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2178, RRID:AB_1579194
- Product name
- PRMT7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRMT7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.
PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity.
The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Lee JH, Cook JR, Yang ZH, Mirochnitchenko O, Gunderson SI, Felix AM, Herth N, Hoffmann R, Pestka S
The Journal of biological chemistry 2005 Feb 4;280(5):3656-64
The Journal of biological chemistry 2005 Feb 4;280(5):3656-64
PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity.
Miranda TB, Miranda M, Frankel A, Clarke S
The Journal of biological chemistry 2004 May 28;279(22):22902-7
The Journal of biological chemistry 2004 May 28;279(22):22902-7
The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Frankel A, Yadav N, Lee J, Branscombe TL, Clarke S, Bedford MT
The Journal of biological chemistry 2002 Feb 1;277(5):3537-43
The Journal of biological chemistry 2002 Feb 1;277(5):3537-43
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT7 (arrow) using PRMT7 polyclonal antibody (Cat # PAB2178).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
