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antibody from Invitrogen Antibodies
Targeting: PRMT7
FLJ10640, KIAA1933
Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-30748 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3. Predicted reactivity: Mouse (80%), Rat (81%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT7 Polyclonal Antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 Polyclonal Antibody (Product # PA5-30748) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 µg of either control rabbit IgG or PRMT7 Polyclonal Antibody (Product # PA5-30748). The precipitated DNA was detected by PCR with primer set targeting to Apex2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT7 Polyclonal Antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 µg of PRMT7 Polyclonal Antibody (Product # PA5-30748) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 Polyclonal Antibody (Product # PA5-30748) diluted at 1:1,000.
