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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-30748 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRMT7 Polyclonal Antibody (Product # PA5-30748). Sample (30 µg of whole cell lysate). Lane A: PC-3. 7.5% SDS PAGE. PRMT7 Polyclonal Antibody (Product # PA5-30748) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT7 Polyclonal Antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 Polyclonal Antibody (Product # PA5-30748) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7 (Product # PA5-30748) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7 (Product # PA5-30748) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7 (Product # PA5-30748) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 µg of either control rabbit IgG or PRMT7 Polyclonal Antibody (Product # PA5-30748). The precipitated DNA was detected by PCR with primer set targeting to Apex2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT7 Polyclonal Antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 µg of PRMT7 Polyclonal Antibody (Product # PA5-30748) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 Polyclonal Antibody (Product # PA5-30748) diluted at 1:1,000.
