Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-24210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM22 Monoclonal Antibody (498805)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, no cross-reactivity with recombinant human ADAM8, 9, 10, 12, 15, 19, 23, 32, or 33 is observed.
- Antibody clone number
- 498805
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human ADAM22 Monoclonal Antibody (Product # MA5-24210) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for ADAM22 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADAM22 in human brain (motor cortex) tissue, human brain (cerebellum) tissue, Jurkat human acute T cell leukemia cell line (negative control), and HepG2 human hepatocellular carcinoma cell line (negative control). Samples were incubated in ADAM22 monoclonal antibody (Product # MA5-24210) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. Specific bands were detected for ADAM22 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions.
