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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-49140 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMPD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Inhibition of nSMase2 Reduces the Transfer of Oligomeric α-Synuclein Irrespective of Hypoxia.
Sackmann V, Sinha MS, Sackmann C, Civitelli L, Bergström J, Ansell-Schultz A, Hallbeck M
Frontiers in molecular neuroscience 2019;12:200
Frontiers in molecular neuroscience 2019;12:200
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMPD3 in HepG2 cell line lysates (35 µg/lane). Lysates were probed with a SMPD3 Antibody (N-term) (Product # PA5-49140).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 nSMase2 inhibition was achieved by nSMase2 siRNA and KO in donor cells. (A-C) Western blot analysis of nSMase2 under (A) normoxia and (B) hypoxia with (C) bar graphs showing its expression relative to beta-actin expression ( n = 3). (D) nSMase enzymatic activity was determined by a colorimetric assay kit using cell lysates ( n = 5). (E) Cytotoxicity of nSMase2 KO and siRNA in cells treated with alpha-syn under normoxia or hypoxia, as determined by LDH activity ( n = 6). The data are presented as the mean +- SEM. * p
