LS-C287187

antibody from LSBio

Targeting: UBE2A HHR6A, HR6A, RAD6A, UBC2

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C287187

Provider

LSBio

Product name

UBE2A Antibody (N-Terminus) LS-C287187

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T cells. Antibody: Affinity purified rabbit anti-Rad6 antibody used at the indicated concentrations. Detection: Chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Rad6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human and Mouse Rad6 by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Rad6 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.