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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-29940 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brain. Predicted reactivity: Mouse (100%), Rat (93%), Zebrafish (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deficiency in the Ubiquitin Conjugating Enzyme UBE2A in Alzheimer's Disease (AD) is Linked to Deficits in a Natural Circular miRNA-7 Sponge (circRNA; ciRS-7).
Zhao Y, Alexandrov PN, Jaber V, Lukiw WJ
Genes 2016 Dec 5;7(12)
Genes 2016 Dec 5;7(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2A Polyclonal Antibody (Product # PA5-29940). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 15% SDS PAGE. UBE2A Polyclonal Antibody (Product # PA5-29940) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UBE2A Polyclonal Antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UBE2A protein stained by UBE2A Polyclonal Antibody (Product # PA5-29940) diluted at 1:500. Blue: Hoechst 33342 staining.
