PA5-30871

antibody from Invitrogen Antibodies

Targeting: LIN7A LIN-7A, MALS-1, TIP-33, VELI1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30871

Provider

Invitrogen Antibodies

Product name

LIN7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The loss of DLG2 isoform 7/8, but not isoform 2, is critical in advanced staged neuroblastoma.

Keane S, Martinsson T, Kogner P, Ejeskär K
Cancer cell international 2021 Mar 16;21(1):170

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In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Ouyang X, Dong C, Ubogu EE
Journal of the peripheral nervous system : JPNS 2019 Jun;24(2):195-206

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Heterodimerization with the β(1) subunit directs the α(2) subunit of nitric oxide-sensitive guanylyl cyclase to calcium-insensitive cell-cell contacts in HEK293 cells: Interaction with Lin7a.

Hochheiser J, Haase T, Busker M, Sömmer A, Kreienkamp HJ, Behrends S
Biochemical pharmacology 2016 Dec 15;122:23-32

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LIN7A Polyclonal Antibody detects LIN7A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A Polyclonal Antibody (Product # PA5-30871) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LIN7A Polyclonal Antibody detects LIN7A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A Polyclonal Antibody (Product # PA5-30871) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Restrictive junctional complex and transporter proteins. Representative indirect fluorescent digital photomicrographs of cryostat axial sections of normal adult sural nerves show UEA-1-positive endothelial cells (green; A, E, I, M, Q, U) with expression of LIN7A, CDH5, CLDN4, VEZT, SLC16A1 and SLC19A2 (red; B, F, J, N, R, V, respectively) restricted to endoneurial microvessels shown in the merged images at lower and higher magnifications (yellow/ orange). LIN7A expression by endoneurial microvessels is apparent only at higher magnification (D). These proteins are expressed by the restrictive blood-nerve barrier and shared with other selected cell types suggesting specialized roles in the restrictive junctional complex formation (LIN7A, CDH5, CLDN4 and VEZT) or specialized nutrient transporters (SLC16A1 and SLC19A2) within normal adult peripheral nerves, as determined by known morphological profiles in situ. White arrows demonstrate positively staining endoneurial microvascular endothelium. Blue (4, 6-diamidino-2-phenylindole) staining indicates nuclei. Scale bar 500 mum for A-C, E-G, I-K, M-O, Q-S and U-W, and 125 mum for D, H, L, P, T and X