PA5-89266

antibody from Invitrogen Antibodies

Targeting: HAS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89266

Provider

Invitrogen Antibodies

Product name

HAS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HRRMRRAGQA LKLPSPRRGS VALCIAAYQE DPDYLRKCLR SAQRISFPDL KVVMVVDGNR QEDAYMLDIF HEVLGGTEQA GFFVWRSNFH EAGEGETEAS LQEGMDRVRD VVRASTFSCI MQKWGGKREV MYTAFKALGD SVDYIQVCDS DTVLDPACTI EMLRVLEEDP QVGGVGGDVQ PPGKGMAVED DQVQAAQVRA TEAWSVHQRH VSREQ; Positive Samples: SKOV3, SW480, Mouse heart, Rat heart, Rat liver; Cellular Location: Membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.18 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HAS3 in extracts of various cell lines using HAS3 Polyclonal Antibody (Product # PA5-89266) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HAS3 was performed in A549 cells using HAS3 Polyclonal Antibody (Product # PA5-89266).