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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-58170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PSDDNPVRAN LRTKGKQYCG KGEDLCANDS YVTIQNSELM SGSMDKGTLG SGSKNNIFYI LLRDWGQNEA ADAMSRLARL APVYLSNRGF SI Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.
Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X
Cell discovery 2022 Jan 18;8(1):3
Cell discovery 2022 Jan 18;8(1):3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3A in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line PC-3. Samples were probed using a POLR3A Polyclonal Antibody (Product # PA5-58170).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3A in human cell lines PC-3 and MCF-7 using a POLR3A Polyclonal Antibody (Product # PA5-58170). Corresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR3A in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a POLR3A Polyclonal Antibody (Product # PA5-58170).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3A in human cerebral cortex using POLR3A Polyclonal Antibody (Product # PA5-58170) shows moderate nuclear positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3A in human testis using POLR3A Polyclonal Antibody (Product # PA5-58170) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3A in human placenta using POLR3A Polyclonal Antibody (Product # PA5-58170) shows moderate to strong positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3A in human liver using POLR3A Polyclonal Antibody (Product # PA5-58170) shows moderate to strong positivity in hepatocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 The BRCT domain of tPARP1 interacts with the Pol III complex. a U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mtPARP1, followed by IP and western blotting with the indicated antibodies. b mtPARP1 or full-length mPARP1 was ectopically expressed in U2OS-PARP1 knockout cells. IP was performed with IgG as a negative control. c U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mTARG1, followed by IP and western blotting with the indicated antibodies. d U2OS-PARP1 knockout cells were transfected with the HA-tagged truncation mutants of mtPARP1 together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel). e U2OS-PARP1 knockout cells were transfected with the indicated combinations of HA-tagged mtPARP1 or mtPARP1 F473A mutant together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel).
