Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-83204 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83204, RRID:AB_11026969
- Product name
- Rabbit Polyclonal POLR3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human POLR3A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: POLR3A Antibody [NBP1-83204] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line PC-3
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POLR3A Antibody [NBP1-83204] - Analysis in human cell lines PC-3 and MCF-7 using Anti-POLR3A antibody. Corresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POLR3A Antibody [NBP1-83204] - Staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POLR3A Antibody [NBP1-83204] - Staining of human liver shows moderate to strong positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POLR3A Antibody [NBP1-83204] - Staining of human placenta shows moderate to strong positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POLR3A Antibody [NBP1-83204] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.