AP5583c

antibody from Abgent

Targeting: STEAP2 IPCA-1, PCANAP1, STAMP1, STMP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP5583c

Provider

Abgent

Proper citation

Abgent Cat#AP5583c, RRID:AB_10816726

Product name

STEA2 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"STEA2 Antibody (Center) (Cat. #AP5583c) western blot analysis in 293 cell line and mouse spleen,liver tissue lysates (15ug/lane).This demonstrates the STEA2 antibody detected the STEA2 protein (arrow)."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of extracts from 293 (lanes 1 and 2),HepG2 (lanes 3 and 4)cell line and mouse spleen (lanes 5 and 6)tissue lysate: 1, 3, 5. using STEA2 Antibody (Center)( AP5583c), (1:500). 2, 4, 6. using STEA2 Antibody (Center), preincubated with the control peptide antigen."

Primary Ab dilution

1:500

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

STEA2 Antibody (Center) (Cat. #AP5583c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STEA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

STEA2 Antibody (Center) (Cat. #AP5583c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50