Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F54829 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- STEA2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific STEA2 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the STEA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate carcinoma tissue with STEA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HEK293 cells with STEA2 antibody; Blue=isotype control, Green= STEA2 antibody.