Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX112673 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112673, RRID:AB_10722873
- Product name
- MUL1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 10% SDS PAGE GTX112673 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- A431 whole cell and membrane extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUL1(GTX112673) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- MUL1 antibody detects MUL1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MUL1 stained by MUL1 antibody (GTX112673) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MUL1 antibody (GTX112673) dilution: 1:500.