PA5-29550

antibody from Invitrogen Antibodies

Targeting: MUL1 C1orf166, FLJ12875, GIDE, MAPL, MULAN, RNF218

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29550

Provider

Invitrogen Antibodies

Product name

MUL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, A431 membrane extract.

Concentration

1.88 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MUL1 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MUL1 polyclonal antibody (Product # PA5-29550) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MUL1 was performed by separating 30 µg of A431 whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MUL1 Polyclonal Antibody (Product # PA5-29550) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MUL1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: MUL1 Polyclonal Antibody (Product # PA5-29550) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MUL1 Polyclonal Antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MUL1 Polyclonal Antibody (Product # PA5-29550) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.