45-5900

antibody from Invitrogen Antibodies

Targeting: PPIF Cyp-D, hCyP3

Antibody data

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Validation data

Product number: 45-5900 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cyclophilin F Monoclonal Antibody (E11AE12BD4)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive controls: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, HepG2 cells; Cultured Human embryonic lung-derived fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.
Reactivity: Human, Mouse, Rat, Bovine
Host: Mouse
Isotype: IgG
Antibody clone number: E11AE12BD4
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C, do not freeze

PPIF protein structure - 45-5900 shown in red.

Madungwe NB, Feng Y, Imam Aliagan A, Tombo N, Kaya F, Bopassa JC
American journal of translational research 2020;12(7):3412-3428

Bala S, Calenda CD, Catalano D, Babuta M, Kodys K, Nasser IA, Vidal B, Szabo G
Hepatology communications 2020 Oct;4(10):1487-1501

Feng Y, Madungwe NB, Imam Aliagan AD, Tombo N, Bopassa JC
Biochemical and biophysical research communications 2019 Dec 10;520(3):606-611

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cyclophilin F in isolated mitochondria using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a concentration of 1 µg/mL. Lane 1: Isolated mitochondria from Human heart, 5 µg loaded. Lane 2: Isolated mitochondria from Bovine heart, 1 µg loaded. Lane 3: Isolated mitochondria from Rat heart, 10 µg loaded. Lane 4: Isolated mitochondria from Mouse heart, 10 µg loaded. Lane 5: Isolated mitochondria from HepG2 cells, 20 µg loaded.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of PC-12 (Lane 1), Jurkat (Lane 2), HT-29 (Lane 3), DU-145 (Lane 4), PANC-1 (Lane 5), U-937 (Lane 6), HEK-293 (Lane 7) and tissue extract of Rat Heart (Lane 8). The blot was probed with Anti- Cyclophilin F Monoclonal Antibody (Product # 45-5900, 1 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 22 kDa band corresponding to Cyclophilin F was observed across the cell lines and tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cyclophilin F in MRC5 cells using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a concentration of 1 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5), were fixed, treated for heat-induced antigen retrieval, and permeabilized. Detection was perfomed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/mL).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cyclophilin F in MRC5 cells using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a concentration of 1 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5), were fixed, treated for heat-induced antigen retrieval, and permeabilized. Detection was perfomed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/mL).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Cyclophilin F in HEK293 cells using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a dilution of 1:200. HEK293 cells were fixed with paraformaldehyde, permeabilized with 1% triton X-100, and blocked with 10% goat serum for 1 hour at room temperature. Detection was perfomed by a goat anti-mouse IgG secondary antibody at a 1:300 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Cyclophilin F in Human cerebellum using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a concentration of 1 µg/mL. Human cerebellum tissue was formalin fixed and paraffin embedded. Note: immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of Cyclophilin F in HL60 cells using an Cyclophilin F monoclonal antibody (Product # 45-5900) at 1 µg/mL is depicted by the blue line. The red line indicates an isotype control antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 4 FIG. Increased expression of genes involved in necrosis in miR-208a KO mice after acute CCl 4 treatment. WT or miR-208a KO mice received oil (n = 4-5/group) or CCl 4 (n = 6/group) treatment for 48 hours. Whole-liver cell lysates were used to determine the protein levels of Bax (A), CypD (B), PCNA (C), and cyclin D1 (D) by western blot analysis. beta-actin was used as a loading control. Density units are shown in the bar diagram from mice, Bax (n = 4 [oil], n = 6 [CCl 4 ]), CypD (n = 4 [oil]; n = 6 [CCl 4 ]), PCNA (n = 3 [oil], n = 3 [CCl 4 ]), and cyclin D1 (n = 3 [oil], n = 3 [CCl 4 ]). * P < 0.05 versus oil-treated WT mice, # P < 0.05 versus CCl 4 -treated WT mice.