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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX50026 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX50026, RRID:AB_11172567
- Product name
- Cyclophilin F antibody [AT1F5]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
