AM09068PU-S

antibody from OriGene

Targeting: PPIF Cyp-D, hCyP3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AM09068PU-S

Provider

OriGene

Product name

Cyclophilin F (PPIF) mouse monoclonal antibody, clone AT1F5, Purified

Antibody type

Monoclonal

Description

Cyclophilin F (PPIF) mouse monoclonal antibody, clone AT1F5, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

PPIF

Isotype

IgG

Antibody clone number

AT1F5

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis: Cell lysates of 293T and HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF . (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF . (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

IHC