NB120-3446
antibody from Novus Biologicals
Targeting: NFAT5
KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB120-3446 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-3446, RRID:AB_2152629
- Product name
- Rabbit Polyclonal NFAT5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Beta-Glycerophosphate-Induced ORAI1 Expression and Store Operated Ca2+ Entry in Megakaryocytes.
Nucleus pulposus primary cilia alter their length in response to changes in extracellular osmolarity but do not control TonEBP-mediated osmoregulation.
Protective effect of dexmedetomidine against diabetic hyperglycemia-exacerbated cerebral ischemia/reperfusion injury: An in vivo and in vitro study.
Nfat5 is involved in the hyperosmotic regulation of Tmem184b: a putative modulator of ibuprofen transport in renal MDCK I cells.
COX-2 expression mediated by calcium-TonEBP signaling axis under hyperosmotic conditions serves osmoprotective function in nucleus pulposus cells.
Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling.
RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE?
Pelzl L, Sahu I, Ma K, Heinzmann D, Bhuyan AAM, Al-Maghout T, Sukkar B, Sharma Y, Marini I, Rigoni F, Artunc F, Cao H, Gutti R, Voelkl J, Pieske B, Gawaz M, Bakchoul T, Lang F
Scientific reports 2020 Feb 3;10(1):1728
Scientific reports 2020 Feb 3;10(1):1728
Nucleus pulposus primary cilia alter their length in response to changes in extracellular osmolarity but do not control TonEBP-mediated osmoregulation.
Choi H, Madhu V, Shapiro IM, Risbud MV
Scientific reports 2019 Oct 29;9(1):15469
Scientific reports 2019 Oct 29;9(1):15469
Protective effect of dexmedetomidine against diabetic hyperglycemia-exacerbated cerebral ischemia/reperfusion injury: An in vivo and in vitro study.
Chen L, Cao J, Cao D, Wang M, Xiang H, Yang Y, Ying T, Cong H
Life sciences 2019 Oct 15;235:116553
Life sciences 2019 Oct 15;235:116553
Nfat5 is involved in the hyperosmotic regulation of Tmem184b: a putative modulator of ibuprofen transport in renal MDCK I cells.
Rasmussen RN, Christensen KV, Holm R, Nielsen CU
FEBS open bio 2019 Jun;9(6):1071-1081
FEBS open bio 2019 Jun;9(6):1071-1081
COX-2 expression mediated by calcium-TonEBP signaling axis under hyperosmotic conditions serves osmoprotective function in nucleus pulposus cells.
Choi H, Chaiyamongkol W, Doolittle AC, Johnson ZI, Gogate SS, Schoepflin ZR, Shapiro IM, Risbud MV
The Journal of biological chemistry 2018 Jun 8;293(23):8969-8981
The Journal of biological chemistry 2018 Jun 8;293(23):8969-8981
Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling.
Hollborn M, Fischer S, Kuhrt H, Wiedemann P, Bringmann A, Kohen L
Molecular vision 2017;23:116-130
Molecular vision 2017;23:116-130
RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE?
Johnson ZI, Shapiro IM, Risbud MV
The Journal of biological chemistry 2016 Dec 23;291(52):26686-26697
The Journal of biological chemistry 2016 Dec 23;291(52):26686-26697
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing NFAT5 at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFAT5 Antibody [NB120-3446] - Analysis of human NFAT5 from transfected BHK cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed on U2OS cells. The antigen:antibody complex was formed by incubating 500ug whole cell lysate with 3ug of rabbit polyclonal antibody recognizing NFAT5 overnight on a rocking platform at 4C. The immune-complex was captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were washed and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NFAT5 Antibody [NB120-3446] - Normal biopsies of deparaffinized human skeletal muscle tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NFAT5 Antibody [NB120-3446] - Normal biopsies of deparaffinized human brain tissue.