NB120-3446

Pelzl L, Sahu I, Ma K, Heinzmann D, Bhuyan AAM, Al-Maghout T, Sukkar B, Sharma Y, Marini I, Rigoni F, Artunc F, Cao H, Gutti R, Voelkl J, Pieske B, Gawaz M, Bakchoul T, Lang F
Scientific reports 2020 Feb 3;10(1):1728

Nucleus pulposus primary cilia alter their length in response to changes in extracellular osmolarity but do not control TonEBP-mediated osmoregulation.

Choi H, Madhu V, Shapiro IM, Risbud MV
Scientific reports 2019 Oct 29;9(1):15469

Protective effect of dexmedetomidine against diabetic hyperglycemia-exacerbated cerebral ischemia/reperfusion injury: An in vivo and in vitro study.

Chen L, Cao J, Cao D, Wang M, Xiang H, Yang Y, Ying T, Cong H
Life sciences 2019 Oct 15;235:116553

Nfat5 is involved in the hyperosmotic regulation of Tmem184b: a putative modulator of ibuprofen transport in renal MDCK I cells.

Rasmussen RN, Christensen KV, Holm R, Nielsen CU
FEBS open bio 2019 Jun;9(6):1071-1081

COX-2 expression mediated by calcium-TonEBP signaling axis under hyperosmotic conditions serves osmoprotective function in nucleus pulposus cells.

Choi H, Chaiyamongkol W, Doolittle AC, Johnson ZI, Gogate SS, Schoepflin ZR, Shapiro IM, Risbud MV
The Journal of biological chemistry 2018 Jun 8;293(23):8969-8981

Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling.

Hollborn M, Fischer S, Kuhrt H, Wiedemann P, Bringmann A, Kohen L
Molecular vision 2017;23:116-130

RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE?

Johnson ZI, Shapiro IM, Risbud MV
The Journal of biological chemistry 2016 Dec 23;291(52):26686-26697

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing NFAT5 at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: NFAT5 Antibody [NB120-3446] - Analysis of human NFAT5 from transfected BHK cell lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed on U2OS cells. The antigen:antibody complex was formed by incubating 500ug whole cell lysate with 3ug of rabbit polyclonal antibody recognizing NFAT5 overnight on a rocking platform at 4C. The immune-complex was captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were washed and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: NFAT5 Antibody [NB120-3446] - Normal biopsies of deparaffinized human skeletal muscle tissue.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: NFAT5 Antibody [NB120-3446] - Normal biopsies of deparaffinized human brain tissue.