11996-1-AP

antibody from Proteintech Group

Targeting: MAP3K11 MEKK11, MLK3, PTK1, SPRK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11996-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11996-1-AP, RRID:AB_10694137

Product name

MLK3 antibody

Antibody type

Polyclonal

Description

KD/KO validated MLK3 antibody (Cat. #11996-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy.

Huang J, Liu Y, Chen JX, Lu XY, Zhu WJ, Qin L, Xun ZX, Zheng QY, Li EM, Sun N, Xu C, Chen HY
Acta pharmacologica Sinica 2022 Jan;43(1):50-63

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Silence of MLK3 alleviates lipopolysaccharide-induced lung epithelial cell injury via inhibiting p53-mediated ferroptosis.

Chen X, Qi G, Fang F, Miao Y, Wang L
Journal of molecular histology 2022 Apr;53(2):503-510

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Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.

Ying C, Dai J, Fan G, Zhou Z, Gan T, Zhang Y, Song Y, Zhou X
Frontiers in physiology 2021;12:679166

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Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload.

Wang J, Deng B, Liu J, Liu Q, Guo Y, Yang Z, Fang C, Lu L, Chen Z, Xian S, Wang L, Huang Y
Journal of ethnopharmacology 2021 Jun 28;274:114078

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Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.

Wang J, Deng B, Liu Q, Huang Y, Chen W, Li J, Zhou Z, Zhang L, Liang B, He J, Chen Z, Yan C, Yang Z, Xian S, Wang L
Cell death & disease 2020 Jul 24;11(7):574

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High-Throughput Screening Identifies Mixed-Lineage Kinase 3 as a Key Host Regulatory Factor in Zika Virus Infection.

Xu H, Cheng M, Chi X, Liu X, Zhou J, Lin T, Yang W
Journal of virology 2019 Sep 15;93(18)

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Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

Yin M, Shen Z, Yang L, Zheng W, Song H
International journal of molecular medicine 2019 May;43(5):2086-2102

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 11996-1-AP(MAP3K11 antibody) at dilution of 1:200

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 11996-1-AP(MAP3K11 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue