PA5-44216

antibody from Invitrogen Antibodies

Targeting: GLO1 GLOD1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-44216

Provider

Invitrogen Antibodies

Product name

GLO1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: TMLRVKDPKK SLDFYTRVLG MTLIQKCDFP IMKFSLYFLA YEDKNDIPKE

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human THP-1 cell lysate using an anti-GLO1 polyclonal antibody (Product # PA5-44216).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GLO1 Polyclonal Antibody (Product # PA5-44216) and a 21 kDa band corresponding to GLO1 was observed in Mouse Liver and Rat Liver and not in Mouse Skeletal Muscle and Mouse Spleen tissue lysates which is reported to be negative. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Spleen (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).