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- Western blot [1]
- Flow cytometry [1]
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- Product number
- NBP2-59369 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glyoxalase I Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Aanalysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal-BSA using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369] - Analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.
