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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34623 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLO1 Recombinant Rabbit Monoclonal Antibody (JU44-11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Mouse testis tissue lysate, C2C12, 293, rat spleen tissue lysate, 293T, HUVEC, SH-SY5Y, Hela, rat epididymis tissue, human thyroid gland tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU44-11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLO1 in Lane 1: Mouse testis tissue, Lane 2: C2C12, Lane 3: 293, Lane 4: Rat spleen tissue. Samples were incubated with GLO1 monoclonal antibody (Product # MA5-34623), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLO1 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GLO1 monoclonal antibody (Product # MA5-34623), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLO1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GLO1 monoclonal antibody (Product # MA5-34623), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLO1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GLO1 monoclonal antibody (Product # MA5-34623), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLO1 in paraffin-embedded rat epididymis tissue. Samples were incubated with GLO1 monoclonal antibody (Product # MA5-34623), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLO1 in paraffin-embedded human thyroid gland tissue. Samples were incubated with GLO1 monoclonal antibody (Product # MA5-34623), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GLO1 in HeLa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with GLO1 monoclonal antibody (Product # MA5-34623) at a dilution of 1:100.
