PA5-58785

antibody from Invitrogen Antibodies

Targeting: RASSF9 P-CIP1, PAMCI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58785

Provider

Invitrogen Antibodies

Product name

RASSF9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YELLAKEFNS LHISNKDGCQ LKENRAKESE VPSSNGEIPP FTQRVFSNYT NDTDSDTGIS SNHSQDSETT VGDVVLLST Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 84%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.35 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

RASSF9 promotes NSCLC cell proliferation by activating the MEK/ERK axis.

Yuan J, Ju Q, Zhu J, Jiang Y, Yang X, Liu X, Ma J, Sun C, Shi J
Cell death discovery 2021 Jul 31;7(1):199

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RASSF9 using RASSF9 Polyclonal Antibody (Product # PA5-58785) (A) shows similar pattern to an independent RASSF9 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RASSF9 in mouse cell line NIH-3T3 and rat cell line NBT-II using a RASSF9 Polyclonal Antibody (Product # PA5-58785).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RASSF9 in human rectum using a RASSF9 Polyclonal Antibody (Product # PA5-58785) shows moderate cytoplasmic positivity in glandular cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 RASSF9 is highly expressed in NSCLC and plays a role in stimulating tumor cell proliferation. a The mRNA levels of RASSF9 in normal and NSCLC tissues. n = 10. b The mRNA levels of RASSF9 in human bronchial epithelial cells (16HBE) and NSCLC tumor cell lines (A549, H1299, and H1650). c RASSF9 was increased by the transfection with the plasmid carrying Rassf9 . The control cells were transfected with the empty vector pcDNA3.1. Twenty-four hours post-transfection, cells were harvested for analyzing RASSF9 by western blot. Actin was used as a loading control. d RASSF9 improves cell viability. A549, H1299, and H1650 cells were transfected with plasmid expressing Rassf9 and cell viability was analyzed by the method of MTT at different time points as indicated. The control cells were transfected with the empty vector pcDNA3.1 (EV). e RASSF9 promotes cell proliferation. A549, H1299, and H1650 cells were transfected with the plasmid expressing Rassf9 . Twenty-four hours post-transfection, cell proliferation was analyzed by EdU incorporation assay. The control cells were transfected with the empty vector pcDNA3.1 (EV). Scale bar = 500 mum. f Quantitative analysis of EdU incorporation. Data are presented as means +- SD (error bars). * P < 0.05, ** P < 0.01, and *** P < 0.001. Student's t -test.