Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-77357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Connexin 45 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.
Dai W, Nadadur RD, Brennan JA, Smith HL, Shen KM, Gadek M, Laforest B, Wang M, Gemel J, Li Y, Zhang J, Ziman BD, Yan J, Ai X, Beyer EC, Lakata EG, Kasthuri N, Efimov IR, Broman MT, Moskowitz IP, Shen L, Weber CR
Circulation research 2020 Jul 3;127(2):e28-e43
Circulation research 2020 Jul 3;127(2):e28-e43
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4) with Connexin 45 polyclonal antibody (Product # PA5-77357) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4) with Connexin 45 polyclonal antibody (Product # PA5-77357) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Connexin 45 in perfusion-fixed, frozen mouse brain. A) Samples were probed with Connexin 45 polyclonal antibody (Product # PA5-77357) using a dilution of 1:400, and incubated with anti-rabbit -Cy3 and DAPI. Images also show stained DAPI.
