Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- RQ7520 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NOB1 Antibody / ART-4
- Antibody type
- Polyclonal
- Description
- This highly specific NOB1 antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NOB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of ) human HeLa, 2) human HepG2, 3) human K562, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with NOB1 antibody. Predicted molecular weight ~47 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.