Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- Ab095317 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD79a Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human CD79a Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.ApplicationWB: 0.5-2 µg/mLIHC: 5-20 µg/mLFlow: 20_g/mLProtein FunctionRequired in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 36-219
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD79a Antibody (Ab095317) - Western Blot All lanes: CD79a Antibody (Ab095317) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 25 kDa Observed band size: 45 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD79a Antibody (Ab095317) - IHC DAB staining on IHC Samples: Human Lymphoma Tissue Primary Ab: 10 µg/mL CD79a Antibody (Ab095317) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD79a Antibody (Ab095317) - Flow Cytometry Raji human Burkitt‘s lymphoma cell line was stained with CD79a Antibody (Ab095317) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody.