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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00002034-M08 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002034-M08, RRID:AB_10901799
- Product name
- EPAS1 monoclonal antibody (M08), clone 1G2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant EPAS1.
- Antigen sequence
SKETEVFYELAHELPLPHSVSSHLDKASIMRLAIS
FLRTHKLLSSVCSENESEAEADQQMDNLYLKALEG
FIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIF
DFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERD
FFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVY
NNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDI
PLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEEL
LGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQY
RMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNY
VLSEI- Isotype
- IgG
- Antibody clone number
- 1G2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EPAS1 expression in transfected 293T cell line by EPAS1 monoclonal antibody (M08), clone 1G2.Lane 1: EPAS1 transfected lysate (Predicted MW: 96.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EPAS1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
