A700-003

antibody from Invitrogen Antibodies

Targeting: EPAS1 bHLHe73, HIF2A, HLF, MOP2, PASD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-003

Provider

Invitrogen Antibodies

Product name

HIF2-alpha Recombinant Rabbit Monoclonal Antibody (BL-95-1A2)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BL-95-1A2

Vial size

100 µL

Concentration

0.40 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha by western blot. Samples: Whole cell lysate (50 µg) from HepG2 and 786-O cells treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (Product # A700-003 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha by WB of immunoprecipitates from HepG2 lysate treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha by WB of HepG2, 293T, and 786-O lysate treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha in FFPE 786-O cells by ICC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (Product # A700-003 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha in FFPE Hep-G2 cells with (left) and without (right) cobalt treatment by ICC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (Product # A700-003 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha in FFPE HepG2 cells untreated (left) and treated with CoCl2 (right) by ICC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha (red) in formaldehyde-fixed HepG2 cells untreated (left) and treated with CoCl2 (right) by ICC-IF. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003). Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-201D4). Counterstain: Phalloidin Alexa Fluor® 488 conjugated (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (Product # A700-003 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of human HIF2-alpha binding sites in immunoprecipitates from subcutaneous tumor 786-O cells by ChIP-Seq. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal [BL-95-1A2] (A700-003).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HIF2-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells with 200 µM CoCl2 ( +) or mock treated (-). Antibodies: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (Product # A700-003 lot 3) used for IP at 20 µL/mg lysate. HIF2-alpha was also immunoprecipitated by a previous lot of this antibody (A700-003 lot 2). For blotting immunoprecipitated HIF2-alpha (Product # A700-003) was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.