APRab10504

Targeting: EPAS1 bHLHe73, HIF2A, HLF, MOP2, PASD2

Western blot

ELISA

Antibody data

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Validation data

Product number: APRab10504 - Provider product page
Provider: EnkiLife Biotech Co., Ltd.
Product name: EPAS-1 Rabbit Polyclonal Antibody
Antibody type: Polyclonal
Description: Affinity purification
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: Polyclonal
Vial size: 100 µl
Concentration: 1 mg/ml
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Handling: The antibody solution should be gently mixed before use.

EPAS1 protein structure - APRab10504 shown in red.

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Supportive validation

Submitted by: EnkiLife Biotech Co., Ltd. (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.