Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-53594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir3.4 (KCNJ5) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VTPWDPKKIP KQARDYVPIA TDRTRLLAEG KKPRQRYMEK SGKCNVHHGN VQETY Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references USP8 mutations in corticotroph adenomas determine a distinct gene expression profile irrespective of functional tumour status.
Bujko M, Kober P, Boresowicz J, Rusetska N, Paziewska A, Dąbrowska M, Piaścik A, Pękul M, Zieliński G, Kunicki J, Bonicki W, Ostrowski J, Siedlecki JA, Maksymowicz M
European journal of endocrinology 2019 Dec;181(6):615-627
European journal of endocrinology 2019 Dec;181(6):615-627
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Kir3.4 (KCNJ5) in control (vector only transfected HEK293T lysate) and kCNJ5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-53594).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Kir3.4 (KCNJ5) in human adrenal gland and cerebral cortex tissues using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-53594). Corresponding KCNJ5 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Kir3.4 (KCNJ5) in human adrenal gland using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-53594) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Kir3.4 (KCNJ5) in human cerebral cortex using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-53594) shows low expression as expected.