PA5-119662

antibody from Invitrogen Antibodies

Targeting: KCNJ5 CIR, GIRK4, KATP1, Kir3.4, LQT13

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119662

Provider

Invitrogen Antibodies

Product name

Kir3.4 (KCNJ5) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue. Subcellular Location: Membrane.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Kir3.4 on different lysates using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662) at 1:500 dilution. Positive control: Lane 1: Mouse heart ; Lane 2: Rat heart.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in 293T cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in HepG2 cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in LOVO cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat heart tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human lung cancer tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human pancreas tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse pancreas tissue using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Kir3.4 (KCNJ5) in HepG2 cells using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662) at 1:100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.