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Western blot
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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA017353 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA017353, RRID:AB_1852135
- Product name
- Anti-KCNJ5
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human KCNJ5, Gene description: potassium inwardly-rectifying channel, subfamily J, member 5, Alternative Gene Names: CIR, GIRK4, KATP1, Kir3.4, LQT13, Validated applications: WB, IHC, Uniprot ID: P48544, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Targeted disruption of the Kcnj5 gene in the female mouse lowers aldosterone levels
DACH1 , a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor-β Signaling and Suppresses Aldosterone Secretion
Novel Insertion Mutation in KCNJ5 Channel Produces Constitutive Aldosterone Release From H295R Cells
DACH1, a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor- Signaling and Suppresses Aldosterone Secretion
Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension
Hardege I, Long L, Al Maskari R, Figg N, O’Shaughnessy K
Clinical Science 2018;132(1):145-156
Clinical Science 2018;132(1):145-156
DACH1 , a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor-β Signaling and Suppresses Aldosterone Secretion
Zhou J, Shaikh L, Neogi S, McFarlane I, Zhao W, Figg N, Brighton C, Maniero C, Teo A, Azizan E, Brown M
Hypertension 2015;65(5):1103-1110
Hypertension 2015;65(5):1103-1110
Novel Insertion Mutation in KCNJ5 Channel Produces Constitutive Aldosterone Release From H295R Cells
Hardege I, Xu S, Gordon R, Thompson A, Figg N, Stowasser M, Murrell-Lagnado R, O'Shaughnessy K
Molecular Endocrinology 2015;29(10):1522-1530
Molecular Endocrinology 2015;29(10):1522-1530
DACH1, a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor- Signaling and Suppresses Aldosterone Secretion
Zhou J, Shaikh L, Neogi S, McFarlane I, Zhao W, Figg N, Brighton C, Maniero C, Teo A, Azizan E, Brown M
Hypertension 2015 April;65(5):1103-1110
Hypertension 2015 April;65(5):1103-1110
Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension
Azizan E, Poulsen H, Tuluc P, Zhou J, Clausen M, Lieb A, Maniero C, Garg S, Bochukova E, Zhao W, Shaikh L, Brighton C, Teo A, Davenport A, Dekkers T, Tops B, Küsters B, Ceral J, Yeo G, Neogi S, McFarlane I, Rosenfeld N, Marass F, Hadfield J, Margas W, Chaggar K, Solar M, Deinum J, Dolphin A, Farooqi I, Striessnig J, Nissen P, Brown M
Nature Genetics 2013;45(9):1055-1060
Nature Genetics 2013;45(9):1055-1060
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and kCNJ5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424464).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human adrenal gland and skeletal muscle tissues using HPA017353 antibody. Corresponding KCNJ5 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
