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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- MA5-37999 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TC10 Recombinant Rabbit Monoclonal Antibody (10F7Y7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: 293T, A-549, 22Rv1, Mouse heart, Rat heart Immunogen sequence: KEEWVPELKE YAPNVPFLLI GTQIDLRDDP KTLARLNDMK EKPICVEQGQ KLAKEIGACC YVECSALTQK GLKTVFDEAI IAILTPKKHT VKKRIGSRCI NCCLIT
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 10F7Y7
- Vial size
- 100 µL
- Concentration
- 1.19 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TC10 in extracts of various cell lines. Samples were incubated with TC10 Monoclonal antibody (Product # MA5-37999) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TC10 in extracts of rat heart. Samples were incubated with TC10 Monoclonal antibody (Product # MA5-37999) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TC10 in paraffin-embedded rat spleen. Samples were incubated with TC10 Monoclonal antibody (Product # MA5-37999) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TC10 in paraffin-embedded rat spleen. Samples were incubated with TC10 Monoclonal antibody (Product # MA5-37999) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
