Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 - ELISA [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - H00222643-M03 - Provider product page

 - Provider
 - Abnova Corporation
 - Proper citation
 - Abnova Corporation Cat#H00222643-M03, RRID:AB_1237898
 - Product name
 - UNC5CL monoclonal antibody (M03), clone 3G7
 - Antibody type
 - Monoclonal
 - Description
 - Mouse monoclonal antibody raised against a full-length recombinant UNC5CL.
 - Antigen sequence
 MCPQESSFQPSQFLLLVGVPVASVLLLAQCLRWHC
PRRLLGACWTLNGQEEPVSQPTPQLENEVSRQHLP
ATLPEMVAFYQELHTPTQGQTMVRQLMHKLLVFSA
REVDHRGGCLMLQDTGISLLIPPGAVAVGRQERVS
LILVWDLSDAPSLSQAQGLVSPVVACGPHGASFLK
PCTLTFKHCAEQPSHARTYSSNTTLLDAKVWRPLG
RPGAHASRDECRIHLSHFSLYTCVLEAPVGREARK
WLQLAVFCSPLVPGQSHLQLRIYFLNNTPCALQWA
LTNEQPHGGRLRGPCQLFDFNGARGDQCLKLTYIS
EGWENVDDSSCQLVPHLHIWHGKCPFRSFCFRRKA
ADENEDCSALTNEIIVTMHTFQDGLETKYMEILRF
QASEEESWAAPPPVSQPPPCNRLPPELFEQLRMLL
EPNSITGNDWRGLASHLGLCGMKIRFLSCQRSPAA
AILELFEEQNGSLQELHYLMTVMERLDCASAIQNY
LSGTHGGSPGPERGGARDNQGLELDEKL- Isotype
 - IgG
 - Antibody clone number
 - 3G7
 - Storage
 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
 
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					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Western Blot analysis of UNC5CL expression in transfected 293T cell line by UNC5CL monoclonal antibody (M03), clone 3G7.Lane 1: UNC5CL transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Detection limit for recombinant GST tagged UNC5CL is 1 ng/ml as a capture antibody.
 - Validation comment
 - Sandwich ELISA (Recombinant protein)
 - Protocol
 - Protocol
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence of monoclonal antibody to UNC5CL on HeLa cell . [antibody concentration 10 ug/ml]
 - Validation comment
 - Immunofluorescence
 - Protocol
 - Protocol