Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50705 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCHR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total SLC1 / MCHR1 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.9 mg/mL
- Storage
- -20°C
Submitted references In vitro Radiopharmaceutical Evidence for MCHR1 Binding Sites in Murine Brown Adipocytes.
Balber T, Benčurová K, Kiefer FW, Kulterer OC, Klebermass EM, Egger G, Tran L, Wagner KH, Viernstein H, Pallitsch K, Spreitzer H, Hacker M, Wadsak W, Mitterhauser M, Philippe C
Frontiers in endocrinology 2019;10:324
Frontiers in endocrinology 2019;10:324
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCHR1 was performed by loading (from left to right): Human fetal liver and testis tissue, 293T and A172 cell, mouse brain and human fetal brain tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a MCHR1 antibody (Product # PA5-50705) at a 1/350 dilution for 1 minute, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCHR1 was performed by loading (from left to right): Human fetal liver and testis tissue, 293T and A172 cell, mouse brain and human fetal brain tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a MCHR1 antibody (Product # PA5-50705) at a 1/350 dilution for 1 minute, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MCHR1 in paraffin embedded Human ovarian cancer tissue using MCHR1 Polyclonal Antibody (Product # PA5-50705) at a 1:30 dilution.