711649

antibody from Invitrogen Antibodies

Targeting: MCHR1 GPR24, MCH1R, SLC1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711649

Provider

Invitrogen Antibodies

Product name

MCHR1 Recombinant Superclonal™ Antibody (20HCLC)

Antibody type

Other

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Mouse, Rabbit. Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

20HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

An N-terminal fusion allele to study melanin concentrating hormone receptor 1.

Jasso KR, Kamba TK, Zimmerman AD, Bansal R, Engle SE, Everett T, Wu CH, Kulaga H, Reed RR, Berbari NF, McIntyre JC
Genesis (New York, N.Y. : 2000) 2021 Aug;59(7-8):e23438

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Melanin-Concentrating Hormone Receptor 1 (MCHR1) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-MCHR1 Recombinant Rabbit Polyclonal Antibody (Product # 711649 at a concentration of 1 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Melanin-Concentrating Hormone Receptor 1 (MCHR1) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-MCHR1 Recombinant Rabbit Superclonal™ Antibody (Product # 711649 at a concentration of 1 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells.