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LearnPA5-109263
antibody from Invitrogen Antibodies
Targeting: ERGIC3
C20orf47, CGI-54, Erv46, NY-BR-84, PRO0989, SDBCAG84
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-109263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERGIC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Siha, human placenta tissue, rat bone marrow tissue, LOVO, human colon cancer tissue, human uterus tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERGI3 in Lane 1: SiHa, Lane 2: Human placenta, Lane 3: Rat bone marrow. Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ERGI3 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ERGI3 polyclonal antibody (Product # PA5-109263), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ERGI3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ERGI3 polyclonal antibody (Product # PA5-109263), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERGI3 in paraffin-embedded mouse brain tissue. Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERGI3 in paraffin-embedded human uterus tissue. Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERGI3 in paraffin-embedded human placenta tissue. Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERGI3 in paraffin-embedded human colon cancer tissue. Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ERGI3 in LOVO cells (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Samples were incubated with ERGI3 polyclonal antibody (Product # PA5-109263) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
