PA5-31996
antibody from Invitrogen Antibodies
Targeting: ERGIC3
C20orf47, CGI-54, Erv46, NY-BR-84, PRO0989, SDBCAG84
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31996 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERGIC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.65 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERGIC3 Polyclonal Antibody (Product # PA5-31996). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 10% SDS PAGE. ERGIC3 Polyclonal Antibody (Product # PA5-31996) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERGIC3 Polyclonal Antibody detects ERGIC3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERGIC3 protein stained by ERGIC3 Polyclonal Antibody (Product # PA5-31996) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ERGIC3 (Product # PA5-31996) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.