Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF5195 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal IL-34 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-34 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL-34 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells.
IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
Interleukin-34 selectively enhances the neuroprotective effects of microglia to attenuate oligomeric amyloid-β neurotoxicity.
Ogawa S, Matsuoka Y, Takada M, Matsui K, Yamane F, Kubota E, Yasuhara S, Hieda K, Kanayama N, Hatano N, Tokumitsu H, Magari M
The Journal of biological chemistry 2019 Feb 15;294(7):2386-2396
The Journal of biological chemistry 2019 Feb 15;294(7):2386-2396
IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
Baek JH, Zeng R, Weinmann-Menke J, Valerius MT, Wada Y, Ajay AK, Colonna M, Kelley VR
The Journal of clinical investigation 2015 Aug 3;125(8):3198-214
The Journal of clinical investigation 2015 Aug 3;125(8):3198-214
CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley ER, Kanayama N, Mabbott NA, Magari M, Ohmori H
Journal of leukocyte biology 2014 Jan;95(1):19-31
Journal of leukocyte biology 2014 Jan;95(1):19-31
Interleukin-34 selectively enhances the neuroprotective effects of microglia to attenuate oligomeric amyloid-β neurotoxicity.
Mizuno T, Doi Y, Mizoguchi H, Jin S, Noda M, Sonobe Y, Takeuchi H, Suzumura A
The American journal of pathology 2011 Oct;179(4):2016-27
The American journal of pathology 2011 Oct;179(4):2016-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IL-34 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-34 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-34 and Neutralization by Mouse IL-34 Antibody. Recombinant Mouse IL-34 (Catalog # 5195-ML) induces proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-34 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5195). The ND50 is typically 0.3-1.5 μg/mL.