LS-C185540

antibody from LSBio

Targeting: SART1 Ara1, HAF, SNRNP110, Snu66

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185540

Provider

LSBio

Product name

SART1 Antibody (aa137-447) LS-C185540

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. SART1 antibody diluted at 1:5000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: Jurkat, B: Raji, C: K562, D: NCI-H929. 7.5% SDS PAGE. SART1 antibody diluted at 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Cal27 xenograft using SART1 antibody at 1:500 dilution.