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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-97120 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SART1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS ARDLQ; Positive Samples: HeLa, Jurkat, HepG2, Mouse liver, Mouse brain, Mouse testis, Rat brain; Cellular Location: Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SART1 in 150 μg extracts of Jurkat cells. Samples were precipitated with 3 μg SART1 Polyclonal antibody (Product # PA5-97120). Western blot was performed from the immunoprecipitate using SART1 Polyclonal antibody (Product # PA5-97120) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SART1 in paraffin-embedded human esophageal. Samples were incubated with SART1 Polyclonal antibody (Product # PA5-97120) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SART1 in paraffin-embedded mouse kidney. Samples were incubated with SART1 Polyclonal antibody (Product # PA5-97120) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SART1 in paraffin-embedded rat brain. Samples were incubated with SART1 Polyclonal antibody (Product # PA5-97120) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of SART1 was performed in 150 µg extracts of Jurkat cells using SART1 Polyclonal Antibody (Product # PA5-97120). Western blot was performed from the immunoprecipitate using SART1 Polyclonal Antibody at a dilution of 1:1000.
