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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-53092 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VRKLREKRQT EGTYRPSSEE QFSHAAEARA PQDSKETVQG CLPI Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Substrate Stiffness Determines the Establishment of Apical-Basal Polarization in Renal Epithelial Cells but Not in Tubuloid-Derived Cells.
Hagelaars MJ, Yousef Yengej FA, Verhaar MC, Rookmaaker MB, Loerakker S, Bouten CVC
Frontiers in bioengineering and biotechnology 2022;10:820930
Frontiers in bioengineering and biotechnology 2022;10:820930
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CRB3 in human cell line U-2 OS shows positivity in cell junctions. Samples were probed using a CRB3 Polyclonal Antibody (Product # PA5-53092).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CRB3 in human duodenum and cerebral cortex tissues using CRB3 Polyclonal Antibody (Product # PA5-53092). Corresponding CRB3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CRB3 in human duodenum using CRB3 Polyclonal Antibody (Product # PA5-53092) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CRB3 in human cerebral cortex using CRB3 Polyclonal Antibody (Product # PA5-53092) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Polarization and junction formation of tubuloid-derived cells on substrates with different elastic moduli. (A) Schematic image depicting the culture of tubuloid-derived cells isolated from cortical human kidney. (B,C) Representative fluorescent images of tubuloid-derived cells cultured on PAA substrates with an elastic modulus of 1, 10 and 50 kPa or on glass substrates. The cells were stained for either Crb3 (red), actin (green) and nuclei (blue) or for Zona Occludens 1 (magenta) and nuclei (blue). Confocal sections are shown in both xy (top) and xz (bottom) direction. Scale bars are 20 um.
