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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF6354 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TREML2/TLT-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse TREML2/TLT-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREML1, rmTREML4, rmTREM1, rmTREM2b, rmTREM3, rmTREM4, and recombinant human TREM2 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q2LA85- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TREML2/TLT-2 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A band was detected for TREML2/TLT-2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TREML2/TLT-2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
