Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47114 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cystatin M Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately 25% cross-reactivity with recombinant mouse Cystatin E/M is observed and less than 1% cross-reactivity with recombinant human (rh) Cystatin A, rhCystatin B, rhCystatin C, rhCystatin D, rhCystatin S, rhCystatin SA, rhCystatin SN, rhFetuin A, and rhFetuin B is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Cystatin M in human skin tissue. Samples were incubated in Cystatin M polyclonal antibody (Product # PA5-47114) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cystatin M in 0.2 mg/mL lysates of human skin tissue. Samples were incubated in Cystatin M polyclonal antibody (Product # PA5-47114) using a dilution of 50 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 1:50. A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.