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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-36599 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDFI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 25 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDFI in Lane 1: SGC7901 whole cell lysate (40 µg), Lane 2: PC3 whole cell lysate (40 µg), Lane 3: HepG2 whole cell lysate (40 µg), Lane 4: HCT116 whole cell lysate (40 µg), Lane 5: C6 whole cell lysate (40 µg), Lane 6: 3T3-L1 whole cell lysate (40 µg). Samples were incubated with MDFI polyclonal antibody (Product # PA5-36599) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDFI using MDFI polyclonal antibody (Product # PA5-36599) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MDFI in PC3 cells. Samples were incubated with MDFI polyclonal antibody (Product # PA5-36599) using a dilution of 1:200.
