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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ7608 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SF3b145 Antibody / SAP145 / Splicing factor 3B subunit 2
- Antibody type
- Polyclonal
- Description
- This highly specific SF3b145 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SF3b145 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human 293T, 4) human K562, 5) rat brain, 6) rat RH35, 7) mouse NIH 3T3 cell lysate with SF3b145 antibody. Predicted molecular weight ~100 kDa but commonly observed at up to ~145 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with SF3b145 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3b145 antibody.
