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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- AF4488 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Porcine 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse 5'-Nucleotidase/CD73 in direct ELISAs and Western blots.
- Reactivity
- Mouse, Porcine
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q61503- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Molecular- and microarray-based analysis of diversity among resting and osteogenically induced porcine mesenchymal stromal cells of several tissue origin.
Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
Bayraktar S, Jungbluth P, Deenen R, Grassmann J, Schneppendahl J, Eschbach D, Scholz A, Windolf J, Suschek CV, Grotheer V
Journal of tissue engineering and regenerative medicine 2018 Jan;12(1):114-128
Journal of tissue engineering and regenerative medicine 2018 Jan;12(1):114-128
Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
Sowa NA, Taylor-Blake B, Zylka MJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Feb 10;30(6):2235-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Feb 10;30(6):2235-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells. 5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- 5'-Nucleotidase/CD73 in Mouse Kidney. 5'-Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of 5'-Nucleotidase/CD73 in Mouse CD4+ Spleno-cytes by Flow Cytometry. Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of 5'-Nucleotidase/CD73 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
