MA5-15537

antibody from Invitrogen Antibodies

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot

Antibody data

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Validation data

Product number: MA5-15537 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD73 Monoclonal Antibody (1D7)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15537 targets NT5E in IHC applications and shows reactivity with Human samples. The MA5-15537 immunogen is purified recombinant fragment of NT5E expressed in E. Coli. MA5-15537 detects NT5E which has a predicted molecular weight of approximately 70kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1D7
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NT5E protein structure - MA5-15537 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD73 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD73 Monoclonal Antibody (1D7) (Product # MA5-15537) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membranous and cytosolic localization. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD73 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD73 Monoclonal Antibody (1D7) (Product # MA5-15537) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membranous and cytosolic localization. The images were captured at 60X magnification.