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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-11162 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphB2 Monoclonal Antibody (48CT12.6.4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 48CT12.6.4
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB2 (arrow) using an EPHB2 monoclonal antibody (Product # MA5-11162) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHB2 gene (Lane 2)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB2 recombinant protein (Fragment) (1:2000) using an EPHB2 monoclonal antibody (Product # MA5-11162).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB2 (arrow) using an EPHB2 monoclonal antibody (Product # MA5-11162) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHB2 gene (Lane 2)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an EPHB2 monoclonal antibody (Product # MA5-11162), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using an EPHB2 monoclonal antibody (Product # MA5-11162) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a PE-conjugated goat anti-mouse secondary antibody.
