MA5-11162

EPHB2 antibody from Invitrogen Antibodies

DRT, EPHT3, ERK, Hek5, Tyro5

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Flow cytometry

Supportive data in Antibodypedia

Provider product page for MA5-11162

Antibody Data

Product number

MA5-11162

Provider

Invitrogen Antibodies

Product name

Anti-EphB2 Monoclonal Antibody (48CT12.6.4)

Provider product page

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

48CT12.6.4

Vial size

200 µL

Concentration

0.4 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EPHB2 recombinant protein (Fragment) (1:2000) using an EPHB2 monoclonal antibody (Product # MA5-11162).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EPHB2 (arrow) using an EPHB2 monoclonal antibody (Product # MA5-11162) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHB2 gene (Lane 2)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an EPHB2 monoclonal antibody (Product # MA5-11162), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an EPHB2 monoclonal antibody (Product # MA5-11162), followed by HRP-conjugated secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HepG2 cells using an EPHB2 monoclonal antibody (Product # MA5-11162) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a PE-conjugated goat anti-mouse secondary antibody.