PA5-18516

antibody from Invitrogen Antibodies

Targeting: WNT3 INT4, MGC131950, MGC138321, MGC138323

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18516

Provider

Invitrogen Antibodies

Product name

WNT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

Wilkerson BA, Zebroski HL, Finkbeiner CR, Chitsazan AD, Beach KE, Sen N, Zhang RC, Bermingham-McDonogh O
eLife 2021 May 18;10

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. Cellular diversity in the nonsensory ampulla epithelium. ( a ) Highlights the position of the nonsensory epithelial cells of the ampulla in UMAP space relative to the whole dataset. ( b ) Cluster analysis in nonsensory ampulla epithelium. ( c ) Expression of markers relative to cell cluster and developmental time. Additional color bars indicate whether markers are known or novel in specificity or implicated in vestibular diseases and dysfunction. ( d ) The relative contributions of cell clusters to the nonsensory ampulla epithelium at the indicated developmental ages. ( e ) Wnt3 RNA ISH localizes to a distinct population of cells in top of the roof of the P1 Swiss Webster ampulla. ( f ) Wnt3 IF in vibratome-sectioned crista localizes to the top of the roof of the P0 B6 ampulla and extends into the semicircular canal. ( g ) Wnt3 IF in a whole mount P7 B6 crista bisected to show the apical surface of the rooftop en face. ( h ) Phalloidin staining in P3 B6 ampulla whole mount. Note that roof cells in the Wnt3 + domain are smaller than surrounding epithelial cells. Brackets in f-g indicate the Wnt3 + rooftop domain. Scale bars = 50 mum. Figure 4--figure supplement 1. Localization of cluster-specific markers in nonsensory ampulla epithelium. RNA in situ hybridization data from the Allen Developing Mouse Brain Atlas is shown for cluster-specific markers in E15.5 cristae. Insets show the cluster specificity in UMAP. Arrowheads indicate expression in the ampulla epithelium.